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mouse embryonic fibroblast conditioned medium  (R&D Systems)


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    R&D Systems mouse embryonic fibroblast conditioned medium
    Mouse Embryonic Fibroblast Conditioned Medium, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblasts+mefs/Mouse+Embryonic+Fibroblast+(MEF)+Conditioned+Media/pm41985336-70-15-29
    Average 93 stars, based on 60 article reviews
    mouse embryonic fibroblast conditioned medium - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis.
    Article Snippet: Cat# 130-102-559; RRID: AB_2657263 Rabbit polyclonal to anti-Eomes Abcam Cat# ab23345; RRID: AB_778267 Rabbit monoclonal to anti-WDR5 Cell Signaling Technology Cat# 13105s; RRID: AB_2620133 Mouse monoclonal to anti-H3K4me3 Abcam Cat# ab1012; RRID: AB_442796 Rabbit polyclonal to anti-H3K27ac Abcam Cat# ab4729; RRID: AB_2118291 Mouse monoclonal to anti-RNA Polymerase II Millipore Cat# 05-623; RRID: AB_309852 Alexa Fluor 488 Donkey Anti-mouse Invitrogen Cat# A21202; RRID: AB_141607 Alexa Fluor 488 Donkey Anti-rabbit Invitrogen Cat# A21206; RRID: AB_141708 Alexa Fluor 594 Donkey Anti-mouse Invitrogen Cat# A21203; RRID: AB_141633 Alexa Fluor 594 Donkey Anti-rabbit Invitrogen Cat# A21207; RRID: AB_141637 Rabbit polyclonal to anti-GCN5 Santa cruz biotechnology Cat# sc-365321X; RRID: AB_10846182 Rabbit polyclonal to anti-GCN5 Santa cruz biotechnology Cat# sc-20698; RRID: AB_2128295 Rabbit polyclonal to anti-H3K4me1 Millipore Cat# 07-436; RRID: AB_310614 Rabbit polyclonal to anti-Nkx2.5 Abcam Cat# ab173204 Mouse monoclonal to anti-cTnT Abcam Cat# ab8295; RRID: AB_306445 Rabbit polyclonal to anti-Mesp1 Abcam Cat# ab173011 Mouse monoclonal to anti-Flag GNI Cat# GNI4110 Rabbit polyclonal to anti-HA-tag Abcam Cat# ab9110; RRID: AB_307019 Rabbit polyclonal to anti-GAPDH Bioworld Cat# AP0063; RRID: AB_2651132 Rabbit polyclonal to anti-GFP Abcam Cat# ab290; RRID: AB_2313768 Mouse monoclonal to PCAF Santa cruz biotechnology Cat# sc-13124; RRID: AB_2128417 Mouse monoclonal to anti-p300 (clone RW128) Millipore Cat# 05-257; RRID: AB_11213111 Rabbit polyclonal to anti-Suz12 Abcam Cat# ab12073; RRID: AB_442939 Bacterial and Virus Strains 5-alpha Competent E. coli TIANGEN CB101 Biological Samples The primary mouse embryonic fibroblasts (MEFs) This paper N/A Chemicals, Peptides, and Recombinant Proteins Recombinant Human BMP-4 Protein R&D Systems Cat# 314-BP Recombinant Human VEGF Protein R&D Systems Cat# 293-VE Recombinant Human Activin A Protein R&D Systems Cat# 338-AC Recombinant Human FGF-basic (bFGF) R&D Systems Cat# 233-FB Recombinant Human FGF-10 Protein R&D Systems Cat# 345-FG L-Ascorbic acids Sigma-Aldrich A5960 Critical Commercial Assays PrimeScript RT reagent Kit Takara Cat# RR037A SYBR Premix Ex Taq Takara Cat# RR420A MEGAshortscript T7 Transcription Kit Invitrogen Cat# AM1354 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Deposited Data Microarray data This paper GEO: GSE111132 (Continued on next page) Cell Stem Cell 22, 1–16.e1–e6, June 1, 2018 e1

    Cell Culture:

    Article Title: Modeling Intestinal Epithelial Function and Polarity using Human iPSCs under Air–Liquid Interface Culture
    Article Snippet: .. Mouse embryonic fibroblasts (MEFs) were seeded onto 6-well plates coated with 20 % Diluted Cultrex ReadyBME, Stem Cell Qualified (R&D Systems), and cultured in DMEM supplemented with 10 % FBS and 1 % PC/SM for 3 days. .. Subsequently, hCSCs were seeded onto the feeder cells and cultured in MimEX GI Expansion Medium (R&D Systems) until the colonies reached 60–80 % confluency.

    Irradiation:

    Article Title: The Wnt pathway induces a naïve-like subpopulation in primed stem cells, while NME7AB leads to a homogeneous naïve-like population.
    Article Snippet: Kevin Yi, JacLeen Nash, Mark Carter, Danica Walkley, Benoit Smagghe, and Andrew Stewart are employees of Minerva Biotechnologies and have shares in the company]. .. PLOS One | https://doi.org/10.1371/journal.pone.0325997 June 25, 2025 3 / 17 irradiated mouse embryonic fibroblasts (MEFs) in 4–10 ng/mL FGF2 (R&D Systems, Minneapolis, MN) and in some cases Rho kinase inhibitor (Y-27632, SelleckChem) was added. ..

    Article Title: Mapping PTBP2 binding in human brain identifies SYNGAP1 as a target for therapeutic splice switching.
    Article Snippet: Cells were transduced with Sendai viral vectors expressing human OCT3/4, SOX2, KLF4, and cMYC (ThermoFisher Scientific) (MOI per manufacturer’s instructions). .. Transduced cells were plated on culture dishes containing irradiated mouse embryonic fibroblasts (MEFs) and maintained in human embryonic stem cell (HES) medium consisting of DMEM/F12 (50:50; Mediatech) supplemented with 20% knock-out serum replacement (ThermoFisher Scientific), 10−4M mercaptoethanol (Sigma), and 10 ng/mL bFGF (R&D Systems). ..

    Modification:

    Article Title: An Elastin-like Polypeptide-fusion peptide targeting capsid-tegument interface as an antiviral against cytomegalovirus infection.
    Article Snippet: .. Mouse embryonic fibroblasts (MEFs) were grown in Dulbecco’s Modification of Eagle’s Medium with 4.5 g/L glucose, l-glutamine and 1 mM sodium pyruvate (DMEM, Corning, catalog number 10-013-CM) containing 10% fetal bovine serum (R&D Systems Biotechne, Flowery Branch, GA, catalog# S11150H), 2 mM l-glutamine and 100 U/mL penicillin–streptomycin (Corning, Manassas, VA; catalog# 30-002-CI) at 37 °C with 5% CO2. ..

    Knock-Out:

    Article Title: Mapping PTBP2 binding in human brain identifies SYNGAP1 as a target for therapeutic splice switching.
    Article Snippet: Cells were transduced with Sendai viral vectors expressing human OCT3/4, SOX2, KLF4, and cMYC (ThermoFisher Scientific) (MOI per manufacturer’s instructions). .. Transduced cells were plated on culture dishes containing irradiated mouse embryonic fibroblasts (MEFs) and maintained in human embryonic stem cell (HES) medium consisting of DMEM/F12 (50:50; Mediatech) supplemented with 20% knock-out serum replacement (ThermoFisher Scientific), 10−4M mercaptoethanol (Sigma), and 10 ng/mL bFGF (R&D Systems). ..



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